Executive Summary: Ecologists have long been challenged to understand the role of host-associated microbial communities in host health, pathogen defense, and co-evolution of hosts and their symbionts. Recent technological advances in high throughput sequencing have allowed scientists to observe the immense microbial diversity of host-associated habitats. However, we are only beginning to appreciate evolutionary interactions of hosts and their microbiomes.
Also visit:
https://www.danforthlab.entomology.cornell.edu/people/jordan-kueneman/
Bee Microbial Diversity |
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Bees are vital members of nearly all ecosystems, and it has been established that their microbial associations are essential for their development and survival. Due to their crucial ecosystem services investigations into how bees acquire, maintain and transmit these microbial associations to the next generation is an area of rapid investigation. Investigations into bee-microbial associations also offer opportunities inform upon host-microbial associations more broadly. Indeed, the grand diversity of bees allows us to uncover evolutionary strategies to promote beneficial microbes and suppress pathogenic microbes to maintain ‘public health’ for group living. I study these attributes of bee biology in the context of the microbial associations.
Pollinator Microbiology
I am beginning to explore the microbiome of moth Panama to better understand how insects move microbes around the forest.
I am beginning to explore the microbiome of moth Panama to better understand how insects move microbes around the forest.
Amphibian Microbiology
The ability to mitigate diseases of host organisms through biological manipulations of the host’s biotic community is a new research area for wildlife disease managers. One understudied area is how amphibian skin-associated microbial communities interact with a known fungal pathogen and how they may function to protect the host from developing disease. Amphibians and diverse bacteria and fungi that inhabit their skin provide an excellent model system for studying interactions between hosts, their microbial communities, and pathogens. Amphibian skin can be repeatedly and non-destructively sampled, and can be manipulated through antibiotics or bioaugmentation.
This system also offers an opportunity to develop therapeutic tools for one of the world’s most threatened taxa. My research on amphibian skin microbiota addresses vital questions about skin microbial diversity at the local and global scale. I am a studying the function of skin microbiota through amphibian development and the consequences of environmental change on these functions.
Plant Leaf Microbiology
In a collaborative effort with Smithsonian Tropical Research Institute, Beijing Genomics Institute, Dow duPont and several American University's, we are beginning to investigate interactions of secondary metabolomics, and microbiota from tree species at the hemispheric scale. This is a major undertaking and there will be updates in the future.
In a collaborative effort with Smithsonian Tropical Research Institute, Beijing Genomics Institute, Dow duPont and several American University's, we are beginning to investigate interactions of secondary metabolomics, and microbiota from tree species at the hemispheric scale. This is a major undertaking and there will be updates in the future.
See Current Publications: Link to Google scholar Page
27) Hammer TJ, Kueneman JG, Argueta-Guzmán M, McFrederick QS, Grant L, Wcislo W, Buchmann S, Danforth BN. Bee breweries: the unusually fermentative, lactobacilli-dominated brood cell microbiomes of cellophane bees. Frontiers in Microbiology.;14:915.
26) Espinoza AC, Urban-Mead KR, Buckner MA, Flórez-Gómez N, Kueneman JG, Danforth BN. Biology of Andrena (Callandrena Sensu Lato) Asteris Robertson (Hymenoptera: Andrenidae), an Eastern Aster Specialist that Makes a Very Deep Nest. Northeastern Naturalist. 2023 Jan;29(4):474-91.
25) Williams CE, Kueneman JG, Nicholson DJ, Rosso AA, Folfas E, Casement B, Gallegos-Koyner MA, Neel LK, Curlis JD, McMillan WO, Cox CL. Sustained Drought, but Not Short-Term Warming, Alters the Gut Microbiomes of Wild Anolis Lizards. Applied and Environmental Microbiology. 2022 Oct 11;88(19):e00530-22.
24) Kueneman JG, Bletz M, Becker M, Gratwicke B, Garcés OA, Hertz A, Holden WM, Ibáñez R, Loudon A, McKenzie V, Parfrey L. Effects of captivity and rewilding on amphibian skin microbiomes. Biological Conservation. 2022 Jul 1;271:109576.
23) Kueneman JG, Gillung J, Van Dyke MT, Fordyce RF, Danforth BN. Solitary bee larvae modify bacterial diversity of pollen provisions in the stem-nesting bee, Osmia cornifrons (Megachilidae). Frontiers in Microbiology. 2022;13.
22) Chen MY, Kueneman JG, González A, Humphrey G, Knight R, McKenzie VJ. Predicting fungal infection rate and severity with skin‐associated microbial communities on amphibians. Molecular Ecology. 2022 Apr;31(7):2140-56.
21) Leray M, Wilkins LG, Apprill A, Bik HM, Clever F, Connolly SR, De León ME, Duffy JE, Ezzat L ... Kueneman JG ... Natural experiments and long-term monitoring are critical to understand and predict marine host–microbe ecology and evolution. PLoS biology. 2021 Aug 19;19(8):e3001322.
20) Sedio BE, Spasojevic MJ, Myers JA, Wright SJ, Person MD, Chandrasekaran H, Dwenger JH, Prechi ML, Lopez CA, Allen DN... Kueneman JG ... Chemical similarity of co-occurring trees decreases with precipitation and temperature in North American forests.
19) Kueneman JG, Esser HJ, Weiss SJ, Jansen PA, Foley JE. Tick microbiomes in neotropical forest fragments are best explained by tick-associated and environmental factors rather than host blood source. Applied and environmental microbiology. 2021 Mar 11;87(7):e02668-20.
18) Woodhams DC, Bletz MC, Becker CG, Bender HA, Buitrago-Rosas D, Diebboll H, Huynh R, Kearns PJ, Kueneman JG, Kurosawa E, LaBumbard BC. Host-associated microbiomes are predicted by immune system complexity and climate. Genome biology. 2020 Dec;21(1):1-20.
17) Kueneman JG, Bletz MC, McKenzie VJ, Becker CG, Joseph MB, Abarca JG, Archer H, Arellano AL, Bataille A, Becker M, Belden LK. Community richness of amphibian skin bacteria correlates with bioclimate at the global scale. Nature Ecology & Evolution. 2019 Mar;3(3):381-9.
16) Flechas SV, Acosta-González A, Escobar LA, Kueneman JG, Sánchez-Quitian ZA, Parra-Giraldo CM, Rollins-Smith LA, Reinert LK, Vredenburg VT, Amézquita A, Woodhams DC. Microbiota and skin defense peptides may facilitate coexistence of two sympatric Andean frog species with a lethal pathogen. The ISME Journal. 2019 Feb;13(2):361-73.
15) McKenzie VJ, Kueneman JG, Harris RN. Probiotics as a tool for disease mitigation in wildlife: insights from food production and medicine. Annals of the New York Academy of Sciences. 2018 Oct;1429(1):18-30.
14) Prest TL, Kimball AK, Kueneman JG, McKenzie VJ. Host‐associated bacterial community succession during amphibian development. Molecular ecology. 2018 Apr;27(8):1992-2006.
13) Kueneman JG, Weiss S, McKenzie VJ. Composition of micro-eukaryotes on the skin of the cascades frog (Rana cascadae) and patterns of correlation between skin microbes and Batrachochytrium dendrobatidis. Frontiers in microbiology. 2017 Dec 8;8:2350.
12) Thompson LR, Sanders JG, McDonald D, Amir A, Ladau J, Locey KJ, Prill RJ, Tripathi A, Gibbons SM, Ackermann G...Kueneman JG... A communal catalogue reveals Earth’s multiscale microbial diversity. Nature. 2017 Nov;551(7681):457-63.
11) Barnhart K, Forman ME, Umile TP, Kueneman JG, McKenzie V, Salinas I, Minbiole KP, Woodhams DC. Identification of bufadienolides from the boreal toad, Anaxyrus boreas, active against a fungal pathogen. Microbial ecology. 2017 Nov;74(4):990-1000.
10) Kueneman JG, Woodhams DC, Harris R, Archer HM, Knight R, McKenzie VJ. Probiotic treatment restores protection against lethal fungal infection lost during amphibian captivity. Proceedings of the Royal Society B: Biological Sciences. 2016 Sep 28;283(1839):20161553.
9) Vences M, Lyra ML, Kueneman JG, Bletz MC, Archer HM, Canitz J, Handreck S, Randrianiaina RD, Struck U, Bhuju S, Jarek M. Gut bacterial communities across tadpole ecomorphs in two diverse tropical anuran faunas. The Science of Nature. 2016 Apr;103(3):1-4.
8) Kueneman JG, Woodhams DC, Van Treuren W, Archer HM, Knight R, McKenzie VJ. Inhibitory bacteria reduce fungi on early life stages of endangered Colorado boreal toads (Anaxyrus boreas). The ISME journal. 2016 Apr;10(4):934-44.
7) Woodhams DC, Bletz M, Kueneman JG, McKenzie V. Managing amphibian disease with skin microbiota. Trends in microbiology. 2016 Mar 1;24(3):161-4.
6 )Hyde ER, Navas-Molina JA, Song SJ, Kueneman JG, Ackermann G, Cardona C, Humphrey G, Boyer D, Weaver T, Mendelson III JR, McKenzie VJ. The oral and skin microbiomes of captive komodo dragons are significantly shared with their habitat. Msystems. 2016 Jul 1;1(4):e00046-16.
5) Rebollar EA, Antwis RE, Becker MH, Belden LK, Bletz MC, Brucker RM, Harrison XA, Hughey MC, Kueneman JG, Loudon AH, McKenzie V. Using “omics” and integrated multi-omics approaches to guide probiotic selection to mitigate chytridiomycosis and other emerging infectious diseases. Frontiers in microbiology. 2016:68.
4) Woodhams DC, Alford RA, Antwis RE, Archer H, Becker MH, Belden LK, Bell SC, Bletz ...Kueneman JG.... Antifungal isolates database of amphibian skin‐associated bacteria and function against emerging fungal pathogens: Ecological Archives E096‐059. Ecology. 2015 Feb;96(2):595
3) Kueneman JG, Parfrey LW, Woodhams DC, Archer HM, Knight R, McKenzie VJ. The amphibian skin‐associated microbiome across species, space and life history stages. Molecular ecology. 2014 Mar;23(6):1238-50.
2) Joseph MB, Mihaljevic JR, Arellano AL, Kueneman JG, Preston DL, Cross PC, Johnson PT. Taming wildlife disease: bridging the gap between science and management. Journal of Applied Ecology. 2013 Jun;50(3):702-12.
1) Fierer N, Ferrenberg S, Flores GE, González A, Kueneman JG, Legg T, Lynch RC, McDonald D, Mihaljevic JR, O'Neill SP, Rhodes ME. From animalcules to an ecosystem: application of ecological concepts to the human microbiome. Annual Review of Ecology, Evolution, and Systematics. 2012 Dec 1;43:137-55.
27) Hammer TJ, Kueneman JG, Argueta-Guzmán M, McFrederick QS, Grant L, Wcislo W, Buchmann S, Danforth BN. Bee breweries: the unusually fermentative, lactobacilli-dominated brood cell microbiomes of cellophane bees. Frontiers in Microbiology.;14:915.
26) Espinoza AC, Urban-Mead KR, Buckner MA, Flórez-Gómez N, Kueneman JG, Danforth BN. Biology of Andrena (Callandrena Sensu Lato) Asteris Robertson (Hymenoptera: Andrenidae), an Eastern Aster Specialist that Makes a Very Deep Nest. Northeastern Naturalist. 2023 Jan;29(4):474-91.
25) Williams CE, Kueneman JG, Nicholson DJ, Rosso AA, Folfas E, Casement B, Gallegos-Koyner MA, Neel LK, Curlis JD, McMillan WO, Cox CL. Sustained Drought, but Not Short-Term Warming, Alters the Gut Microbiomes of Wild Anolis Lizards. Applied and Environmental Microbiology. 2022 Oct 11;88(19):e00530-22.
24) Kueneman JG, Bletz M, Becker M, Gratwicke B, Garcés OA, Hertz A, Holden WM, Ibáñez R, Loudon A, McKenzie V, Parfrey L. Effects of captivity and rewilding on amphibian skin microbiomes. Biological Conservation. 2022 Jul 1;271:109576.
23) Kueneman JG, Gillung J, Van Dyke MT, Fordyce RF, Danforth BN. Solitary bee larvae modify bacterial diversity of pollen provisions in the stem-nesting bee, Osmia cornifrons (Megachilidae). Frontiers in Microbiology. 2022;13.
22) Chen MY, Kueneman JG, González A, Humphrey G, Knight R, McKenzie VJ. Predicting fungal infection rate and severity with skin‐associated microbial communities on amphibians. Molecular Ecology. 2022 Apr;31(7):2140-56.
21) Leray M, Wilkins LG, Apprill A, Bik HM, Clever F, Connolly SR, De León ME, Duffy JE, Ezzat L ... Kueneman JG ... Natural experiments and long-term monitoring are critical to understand and predict marine host–microbe ecology and evolution. PLoS biology. 2021 Aug 19;19(8):e3001322.
20) Sedio BE, Spasojevic MJ, Myers JA, Wright SJ, Person MD, Chandrasekaran H, Dwenger JH, Prechi ML, Lopez CA, Allen DN... Kueneman JG ... Chemical similarity of co-occurring trees decreases with precipitation and temperature in North American forests.
19) Kueneman JG, Esser HJ, Weiss SJ, Jansen PA, Foley JE. Tick microbiomes in neotropical forest fragments are best explained by tick-associated and environmental factors rather than host blood source. Applied and environmental microbiology. 2021 Mar 11;87(7):e02668-20.
18) Woodhams DC, Bletz MC, Becker CG, Bender HA, Buitrago-Rosas D, Diebboll H, Huynh R, Kearns PJ, Kueneman JG, Kurosawa E, LaBumbard BC. Host-associated microbiomes are predicted by immune system complexity and climate. Genome biology. 2020 Dec;21(1):1-20.
17) Kueneman JG, Bletz MC, McKenzie VJ, Becker CG, Joseph MB, Abarca JG, Archer H, Arellano AL, Bataille A, Becker M, Belden LK. Community richness of amphibian skin bacteria correlates with bioclimate at the global scale. Nature Ecology & Evolution. 2019 Mar;3(3):381-9.
16) Flechas SV, Acosta-González A, Escobar LA, Kueneman JG, Sánchez-Quitian ZA, Parra-Giraldo CM, Rollins-Smith LA, Reinert LK, Vredenburg VT, Amézquita A, Woodhams DC. Microbiota and skin defense peptides may facilitate coexistence of two sympatric Andean frog species with a lethal pathogen. The ISME Journal. 2019 Feb;13(2):361-73.
15) McKenzie VJ, Kueneman JG, Harris RN. Probiotics as a tool for disease mitigation in wildlife: insights from food production and medicine. Annals of the New York Academy of Sciences. 2018 Oct;1429(1):18-30.
14) Prest TL, Kimball AK, Kueneman JG, McKenzie VJ. Host‐associated bacterial community succession during amphibian development. Molecular ecology. 2018 Apr;27(8):1992-2006.
13) Kueneman JG, Weiss S, McKenzie VJ. Composition of micro-eukaryotes on the skin of the cascades frog (Rana cascadae) and patterns of correlation between skin microbes and Batrachochytrium dendrobatidis. Frontiers in microbiology. 2017 Dec 8;8:2350.
12) Thompson LR, Sanders JG, McDonald D, Amir A, Ladau J, Locey KJ, Prill RJ, Tripathi A, Gibbons SM, Ackermann G...Kueneman JG... A communal catalogue reveals Earth’s multiscale microbial diversity. Nature. 2017 Nov;551(7681):457-63.
11) Barnhart K, Forman ME, Umile TP, Kueneman JG, McKenzie V, Salinas I, Minbiole KP, Woodhams DC. Identification of bufadienolides from the boreal toad, Anaxyrus boreas, active against a fungal pathogen. Microbial ecology. 2017 Nov;74(4):990-1000.
10) Kueneman JG, Woodhams DC, Harris R, Archer HM, Knight R, McKenzie VJ. Probiotic treatment restores protection against lethal fungal infection lost during amphibian captivity. Proceedings of the Royal Society B: Biological Sciences. 2016 Sep 28;283(1839):20161553.
9) Vences M, Lyra ML, Kueneman JG, Bletz MC, Archer HM, Canitz J, Handreck S, Randrianiaina RD, Struck U, Bhuju S, Jarek M. Gut bacterial communities across tadpole ecomorphs in two diverse tropical anuran faunas. The Science of Nature. 2016 Apr;103(3):1-4.
8) Kueneman JG, Woodhams DC, Van Treuren W, Archer HM, Knight R, McKenzie VJ. Inhibitory bacteria reduce fungi on early life stages of endangered Colorado boreal toads (Anaxyrus boreas). The ISME journal. 2016 Apr;10(4):934-44.
7) Woodhams DC, Bletz M, Kueneman JG, McKenzie V. Managing amphibian disease with skin microbiota. Trends in microbiology. 2016 Mar 1;24(3):161-4.
6 )Hyde ER, Navas-Molina JA, Song SJ, Kueneman JG, Ackermann G, Cardona C, Humphrey G, Boyer D, Weaver T, Mendelson III JR, McKenzie VJ. The oral and skin microbiomes of captive komodo dragons are significantly shared with their habitat. Msystems. 2016 Jul 1;1(4):e00046-16.
5) Rebollar EA, Antwis RE, Becker MH, Belden LK, Bletz MC, Brucker RM, Harrison XA, Hughey MC, Kueneman JG, Loudon AH, McKenzie V. Using “omics” and integrated multi-omics approaches to guide probiotic selection to mitigate chytridiomycosis and other emerging infectious diseases. Frontiers in microbiology. 2016:68.
4) Woodhams DC, Alford RA, Antwis RE, Archer H, Becker MH, Belden LK, Bell SC, Bletz ...Kueneman JG.... Antifungal isolates database of amphibian skin‐associated bacteria and function against emerging fungal pathogens: Ecological Archives E096‐059. Ecology. 2015 Feb;96(2):595
3) Kueneman JG, Parfrey LW, Woodhams DC, Archer HM, Knight R, McKenzie VJ. The amphibian skin‐associated microbiome across species, space and life history stages. Molecular ecology. 2014 Mar;23(6):1238-50.
2) Joseph MB, Mihaljevic JR, Arellano AL, Kueneman JG, Preston DL, Cross PC, Johnson PT. Taming wildlife disease: bridging the gap between science and management. Journal of Applied Ecology. 2013 Jun;50(3):702-12.
1) Fierer N, Ferrenberg S, Flores GE, González A, Kueneman JG, Legg T, Lynch RC, McDonald D, Mihaljevic JR, O'Neill SP, Rhodes ME. From animalcules to an ecosystem: application of ecological concepts to the human microbiome. Annual Review of Ecology, Evolution, and Systematics. 2012 Dec 1;43:137-55.